BBI Data Analysis Tutorial

The BBI bioinformatics team is hosting a Monocle 3 tutorial/training to help you navigate your single-cell RNA-seq data. This tutorial is aimed at assisting you with questions and concerns regarding single-cell data analysis using Monocle 3. Although we encourage anyone interested to attend, this__ particular session is mainly for researchers that have little to no experience with the R programming language or analyzing single-cell data and is primarily aimed to assist beginners.__ In the future, we will have further tutorials for intermediate and more advanced skill levels.

The prerequisites for this session are:

1. R & R studio installed
2. Monocle 3 installed

During this session, we will cover the following topics:

1. An introduction to Monocle 3
2. Loading, QC and initial filtering of data
3. Pre-processing data, dimensionality reduction and making a basic UMAP
4. Basic clustering and plotting genes of interest
5. Finding top markers
6. Gene ontology/gene set analysis There will also be some time to address specific questions.

Host: Aishwarya Gogate When: Tuesday, 7th December 2021 from 9am - 11am Where: Zoom meeting

If you plan to attend this tutorial, please RSVP here.

Tutorial material and meeting invite will be sent in a separate email. If you have further questions, please email aishwarya.gogate@seattlechildrens.org

Important note: We will hold special office hours in the week prior to the tutorial for those who need help with R/Monocle3 installations. Please be sure to sign up at least a day in advance to secure a slot. You can sign up for office hours here: Office hours calendar.