Miller Lab Manager Miranda Galey uses the Nanopore PromethION, Oxford Nanopore’s highest-throughput sequencing device
BBI’s Dr. Danny Miller and the UW’s Nanopore Sequencing Core are providing targeted and whole genome long-read sequencing of both DNA and RNA to assist researchers with projects that could benefit from long-read sequencing technology. As new DNA and RNA sequencing technologies are developed that provide a more comprehensive view of genetic variation, it is imperative that researchers and clinicians have access to leading-edge services, such as this.
Miller and colleagues are using a Nanopore PromethION, Oxford Nanopore’s highest-throughput sequencing device. This is a scalable instrument that runs multiple flow cells independently and can sequence a human to ~40x coverage using a single flow cell. While DNA or RNA of any length can be sequenced, most projects generate read lengths of around 20 to 60kb, which are suitable for many applications, including identification of single nucleotide variants and indels, structural variant discovery, and methylation analysis.
A unique aspect of Nanopore sequencing is the ability to do not only cDNA sequencing, but direct RNA sequencing; recent updates have increased the output and accuracy of direct RNA sequencing. Finally, a single cell long-read sequencing protocol was recently released. Labs interested in collaborating with Miller to test this new protocol should reach out.
For additional information, including consultations, price quotes, and submission guidelines, please contact Miller at dm1@uw.edu or visit: UW Nanopore Sequencing Core - UW Research (washington.edu)
